⛓️ ogilo

Contents:

• Installation
  • The easy way
  • From source
• Usage
  • Files
  • Constant sequences
  • Type IIS restriction enzyme sites
  • Output
  • Examples
  • Command line options
• API reference
  • ogilo

Automating construction of oligo library sequences for oligo array synthesis.

ogilo takes your table of sequences (such as a library of guide RNAs) and concatenates them with any other nucleotide sequences you provide, Type IIS restriction sites, and PCR handles.

If your table of sequences contains a column indicating subsets or groups, you can use this column to instruct ogilo to add distinct PCR handles to each group. This allows you to order multiple libraries as one oligo pool and selectively PCR each one as needed.

Source

View source at GitHub.